Modified Diphasic Liver Infusion Agar: Selective Medium for the Axenic Cultivation of Entamoeba histolytica
Published: 2019-10-30
Page: 191-196
Issue: 2019 - Volume 2 [Issue 3]
Zuhair W. Dardona *
Department of Microbiology, Governmental Medical Services, Gaza, Palestine.
Adnan I. Al-Hindi
Department of Medical Parasitology, Islamic University of Gaza, Palestine.
Samia Boussaa
ISPITS-Higher Institute of Nursing and Health Techniques, Ministry of Health, Marrakesh, Morocco.
*Author to whom correspondence should be addressed.
Abstract
A modified diphasic liver infusion agar medium was prepared manually in the laboratory in order to be used for culturing Entamoeba histolytica in vitro. This medium is a good alternative for the commercial one and has the main ingredients for the parasite's growth. E. histolytica was successfully cultured in the present study and PCR technique was performed to confirm Entamoeba species. The growth of E. histolytica trophozoites had started in the third day post-cultivation and reached its highest rate in the seventh. Then the growth started to decrease until the trophozoites completely disappeared after 12-14 days post-cultivation. Cryopreservation using glycerol 10% (V/V) was used in order to preserve trophozoites for later cultivation.
Keywords: E. histolytica, diphasic, liver infusion agar, PCR, trophozoites, cultivation, cryopreservation